1.A continuous reactor is converting
maltose to lactate, acetate and ethanol. The medium contains 40 g.l-1
of maltose and the medium flow rate is 5 liters per hour and the effluent
contains 20 g.l-1 of lactate. What is the productivity of lactate
production from this reactor?
1.
10 Liters Per Hour
2.
20 G Lactate/L
3.
100 G Lactate/H
4.
4 G Lactate/H
2.Transplastomics…..
1.
Offers Little Opportunity For Practical Use
2.
Produces Genes That Are Released In Pollen
3.
Provides Exceptionally Low Yields Of Protein Products
4.
Targets Genes In The Chloroplast
3.KM values of enzyme X
for substrate A and B are 0.1mM and 0.01mM respectively. This suggest that
P) Enzyme X has more affinity towards A
Q) Enzyme X has low affinity towards A
R) Enzyme X has more affinity towards B
S) Enzyme X has low affinity towards B
1. Q, R
2.
Q, S
3.
R, S
4.
P, Q
4.The specific growth rate (µ) of a
microorganism in stationary phase is
1.
0 (Zero)
2.
µ max
3.
less than zero
4.
greater than zero
5.Co-factor
for Glutathione peroxidase is
1.
Mo
2.
Se
3.
Mg
4.
Fe
6.Match each
parameter in group 1 with the appropriate measuring device in group 2
Group 1
Group 2
P.
Pressure 1. Photometer
Q.
Impeller speed 2. Rotameter
R.
Turbidity 3. Diaphragm
gauge
S. Flow rate 4. Tachometer
1.
P-3, Q-4, R-1, S-2
2.
P-1, Q-3, R-2, S-4
3.
P-4, Q-1, R-2, S-3
4.
P-1, Q-2, R-3, S-4
7.A
fed-batch reactor initially contained 2 litre of medium and 1 g.l-1
of substrate. It was fed at 1 litre per hour with a medium containing 1 g.l-1
of substrate. After 10 hours, the concentration of substrate in the reactor was
0.5 g.l-1. The mass of substrate that was used by the culture in the
reactor was
1.
1 G
2.
3 G
3.
6 G
4.
12 G
8.A
knock-out mouse is made using which of the following methods/technologies?
1.
Monoclonal Antibody
2.
Antisense Oligonucleotide
3.
Transgenic
4.
Animal Cloning
9.The
phenomenon in which substrates are used in a sequential manner is known as
1.
Dialysis
2.
Multiplicity
3.
Diauxie
4.
Dialism
10.How
many kilograms of ethanol is produced from 1 kilogram of glucose in ethanol
fermentation?
1.
2.00
2.
0.20
3.
0.51
4.
0.05
11.Molecular
clock of evolution could be traced on basis of
1.
Comparison Of Short Arm Of 16-S RNA
2.
Substitution In Amino Acids Of Polypeptide Due To Mutation
3.
DNA Fingerprinting
4.
Fossil Study
12.Alkaline phosphatase is used
1.
To Remove A Phosphate At The 5`End Of DNA
2.
To Remove A Phosphate At The 3`End Of DNA
3.
To Add A Phosphate At The 5`End Of DNA
4.
To Add A Phosphate At The 3`End Of DNA
13.The
following antibiotics affect the bacterial protein synthesis with their site of
action. Which of the following combinations is correct?
Antibiotic Site of action
P. Streptomycin 1. aminoacyl tRNA association with ribosome
Q. Tetracycline 2. Transpeptidation
R. erythromycin 3. Translocation
S. Chloramphenicol 4. Initiation of protein synthesis
P. Streptomycin 1. aminoacyl tRNA association with ribosome
Q. Tetracycline 2. Transpeptidation
R. erythromycin 3. Translocation
S. Chloramphenicol 4. Initiation of protein synthesis
1.
P-2; Q-3; R-4; S-1
2.
P-4; Q-1; R-3; S-2
3.
P-1; Q-4; R-3; S-2
4.
P-4; Q-1; R-2; S-3
14.A
fermentation system has a kLa of 3 s-1 and a Co*
of 5 ppm of O2. If the bulk liquid is completely depleted of oxygen,
then the oxygen transfer rate will equal to
1.
5 Mg/L/S
2.
10mg/L/S
3.
15 Mg/L/S
4.
Zero
15.In a
Lineweaver-Burk treatment of data, which of the following plots would give you
a straight line of gradient Km/Vmax?
1.
1/V Against [S]
2.
1/V Against 1/[S]
3.
V Against 1/[S]
4.
V Against [S]
16.A
chemostat has a liquid volume of 2 litres and is being fed at a rate of 4
litres per hour. The dilution rate for this reactor would be
1.
2 Per Hour
2.
4 Litres Per Hour
3.
2 Litres
4.
8 Litres Per Square Hour
17.An E. coli strain that requires the amino
acid lysine for growth is termed a/an
1.
Autotroph
2.
Auxotroph
3.
Lysotroph
4.
Prototroph
18.Pseudomonas
aeruginosa has a maximum specific growth rate of 0.8 h-1 when
grown on glucose as a growth limiting substrate. To ensure that the cells do
not washout, the dilution rate must be set to
1.
Equal To 0.8 /H
2.
Greater Than 0.8 /H
3.
Less Than 0.8 /H
4.
Less Than Half The Inverse Of The Residence Time
19.A
strain of Azotobacter is cultured in
a 15 m3 stirred bioreactor
for alginate production. Under operating conditions kLa is 0.17 s-1.
Oxygen solubility in the broth is 8×10-3kg
m-3. The specific rate of
oxygen uptake is 12.5mmol g-1h-1. The Maximum possible cell
concentration is
1.
10 G /L
2.
12 G /L
3.
14 G /L
4.
8 G /L
20.Shine-Dalgarno
sequences are associated with
1.
Transcription
2.
Translation
3.
Replication
4.
Recombination
21. Which of the following would not be expected to result in a dysfunctional protein?
a.Mutation affecting the splice site of an intron
b.Substitution of glycine for alanine at the carboxyl terminus
c.Insertion of two bases in the code for the amino end
d.Nonsense mutation affecting the middle of a potential protein product
e.Deletion of a single base of a codon near the middle of a potential protein
22. Which of the following is a true statement about translation?
a.The genetic code can be overlapping
b.The first nucleotide in a codon has less specificity than the others
c.Only one group of nucleotides codes for each single amino acid
d.Every codon (three nucleotide bases) specifies an amino acid
e.Specific nucleotide sequences signal termination of peptide chains
23. A patient suffers from adenosine deaminase (ADA) deficiency, an autosomal recessive immune deficiency in which bone marrow lymphoblasts cannot replicate to generate immunocompetent lymphocytes. The treatment option that would permanently cure the patient is
a.Germ-line gene therapy to replace one ADA gene copy
b.Germ-line gene therapy to replace both ADA gene copies
c.Somatic cell gene therapy to replace one ADA gene copy in circulating lymphocytes
d.Somatic cell gene therapy to replace both ADA gene copies in circulating lymphocytes
e.Somatic cell gene therapy to replace one ADA gene copy in bone marrow lymphoblasts
24. The proopiomelanocortin (POMC) gene encodes several regulatory proteins that affect pituitary function. In different brain regions, proteins encoded by this gene have different carboxy-terminal peptides. Which of the following best explains the regulatory mechanism?
a.POMC transcription is regulated by different factors in different brain regions
b.POMC translation elongation is regulated by different factors in different brain regions
c.POMC transcription has different enhancers in different brain regions
d.POMC protein undergoes different protein processing in different brain regions
e.POMC protein forms different allosteric complexes in different brain regions
25. A family in which several individuals have arthritis and detached retina is diagnosed with Stickler syndrome. The locus for Stickler syndrome has been mapped near that for type II collagen on chromosome 12, and mutations in the COL2A1 gene have been described in Stickler syndrome. The family became interested in molecular diagnosis to distinguish normal from mildly affected individuals. Which of the results below would be expected in an individual with a promoter mutation at one COL2A1 gene locus?
a.Western blotting detects no type II collagen chains
b.Southern blotting using intronic restriction sites yields normal restriction fragment sizes
c.Reverse transcriptaseñpolymerase chain reaction (RT-PCR) detects one-half normal amounts of COL2A1 mRNA in affected individuals
d.Fluorescent in situ hybridization (FISH) analysis using a COL2A1 probe detects signals on only one chromosome 12
Answer key:
1-b
2-e
3-e
4-d
5-c
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